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  • Direct immunofluorescence on frozen sections of fresh tissue
  • Direct immunofluorescence (DIF) can detect antibody deposits in the skin and mucosae
  • Indirect immunofluorescence, blood
  • Indirect immunofluorescence (IIF) can detect circulating antibodies in the blood


Autoimmune bullous diseases: pemphigus vulgaris, bullous pemphigoid, dermatitis herpetiformis (Duhring’s disease), lupus erythematosus, differential diagnosis of vasculitis (Henoch-Schönlein purpura).

Direct immunofluoroescence

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